Luminol-based Western blot assays use specific antibodies coupled to horseradish peroxidase (HRP) for immunodetection methods. HRP can catalyze the chemiluminescent oxidation of luminol in the presence of hydrogen peroxide. This transformation is used in ultra-sensitive ELISA assays or ECL detection.
Biosynth’s manufacturing process of Luminol (Biosynth Cat.No. L-8600) ensures that highest purity and lot-to-lot consistency is achieved. Therefore it is the product of choice for manufacturers in and high-end immunological assays.
is a substrate for firefly luciferase used for imaging the expression of the luc marker gene and luciferase-fusion conjugates in living cells, tissues and organisms. Luciferin is extensively utilized in reporter gene assays, immunoassays and hygiene monitoring.
D-Luciferin Firefly L-8200
D-Luciferin Firefly, potassium salt, L-8220
D-Luciferin Firefly, sodium salt monohydrate, L-8240
L-Luciferin, potassium salt, L-8280
D-Luciferin 1-(4,5-dimethoxy-2-nitrophenyl)ethyl ester, L-8300
DL-Luciferin 1-(4,5-dimethoxy-2-nitrophenyl)ethyl ester, L-8305
Chemiluminescence and bioluminescence in nature
Chemiluminescence and bioluminescence (the analogous phenomenon in organisms) is observed when a molecule in a chemical reaction changes to an exited state and than emits the excitation energy as a photon. The Chemiluminescence is not an exotic phenomenon, actually a large number of organic materials emit small quantities of photons even at room temperature as a result of oxidation processes. Typically the wavelengths are of emitted light are between 400 - 700 nm, but can extend to the infrared region and UV.
The majority of chemiluminescence processes in nature result from redox reactions. An excitation energy of 160 - 320 kJ/mol is required to emit visible light by chemiluminescence. This energy needs to be available in a single reaction step, within a short time and within a relatively small reaction volume, so it is quenched by secondary reactions. (In reaction media fluorophores may be present that are capable to act as energy acceptors. Those molecules may absorb the excitation energy and show fluorescence light as a result).
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