Inducers are substances that positively regulate the expression of one or more genes. Induction is common in metabolic pathways that result in the catabolism of a substance and the inducer is normally the substrate for the pathway. The effects of inducers are critical to the functioning and sensitivity of many routine assays.
IPTG is an artificial inducer of the lac operon. IPTG binds to the lac repressor and inactivates it, preventing its binding to the lac operator and thus inducing the expression of beta-galactosidase. IPTG cannot be hydrolyzed and broken down by the cell and is therefore called a "gratuitous" inducer.
In molecular cloning procedures, IPTG in combination with X-gal (B-7150) is used for detection of recombinant clones by blue/white screening. (See also LacZ Reporter Gene System)
Isopropyl-beta-D-thioglucuronic acid (IPTGlcA) is a "gratuitous" inducer of beta-glucuronidase, i.e. it is not hydrolyzed by the enzyme, and it very effectively enhances the sensitivity of beta-glucuronidase assays in E. coli by increasing the concentration of the enzyme.
Selection of Biosynth's products
Cat.No. - Product name
E-8900 - Ethyl-beta-D-thiogalactopyranoside
I-8000 - Isopropyl-beta-D-thiogalactopyranoside dioxane free
I-8050 - Isopropyl-beta-D-thiogalactopyranoside dioxane-grade
I-8200 - Isopropyl-beta-D-thioglucopyranoside
I-8350 - Isopropyl-beta-D-thioglucuronic acid, sodium salt
M-3590 - 1-O-Methyl-alpha-D-galactopyranoside
M-3591 - 1-O-Methyl-beta-D-galactopyranoside
M-3592 - 1-O-Methyl-beta-D-glucopyranoside
M-3600 - 1-O-Methyl-beta-D-glucuronic acid, sodium salt
M-4150 - 1-O-Methyl-alpha-D-mannopyranoside
M-4440 - Methyl-beta-D-thiogalactopyranoside
M-4450 - Methyl-beta-D-thioglucopyranoside
M-6600 - 1-O-Methyl-beta-D-xylopyranoside
P-4250 - Phenyl-beta-D-thiogalactopyranoside
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