Chromogenic and Fluorogenic Substrates for the Detection of Enterococci


Enterococcus species are used as indicators of fecal contamination of recreational waters. The U.S. Environmental Protection Agency recommends that the mean number of culturable Enterococci should not exceed 35 cfu per 100 mL over a 30-day period in marine and freshwater environments.

Note our Application Note!

Read more in our comprehensive paper on substrates for Enterococcus detection 

Including: protocols, application examples on both solid and liquid growth media, and suitable test strains

Detection methods

Classically, enterococci are detected using kanamycin-aesculin-azide media. Aesculin is hydrolysed by beta-glucosidase to glucose and aesculetin, the latter forms a brown-black coloured compound with ferric ions.

More recently, chromogenic and fluorogenic substrates for identification of Enterococcus spp. were developed, including a substrate for a second characteristic enzyme of these bacteria, pyroglutamyl aminopeptidase (L-Pyroglutamic acid 7-amido-4-methylcoumarin, Cat. No. P-8500).

Biosynth offers a range of quality substrates for the detection of Enterococci. There are chromogenic and fluorogenic substrates that can be use individually or in combination.

Biosynth chromogenic enzyme substrates for the detection of Enterococci

Cat. No.Enzyme Substrate NameColor
 B-7250  5-Bromo-4-chloro-3-indoxyl-beta-D-glucopyranoside blue color
 B-7270  5-Bromo-6-chloro-3-indoxyl-beta-D-glucopyranoside magenta violet color
 C-5020  6-Chloro-3-indoxyl-beta-D-gluco-pyranoside salmon red color


Biosynth fluorogenic enzyme substrates for the detection of Enterococci

Cat. No.Enzyme Substrate NameFluorescence Color
(in UV light)
 M-5650  4-Methylumbelliferyl beta-D-glucopyranoside blue fluorescence
 P-8500  L-Pyroglutamic acid 7-amido-4-methylcoumarin blue fluorescence

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