Bacteria detection using bioluminescence

Biosynth’s ProLuciferins

Biosynth offers a full range of ProLuciferins (or caged luciferins) for microbiology and bacteria detection. Bioluminescence detection remains unparalleled in terms of sensitivity and selectivity in comparison with chromogenic and fluorogenic methods, lowering significantly the incubation times when detecting a specific species of bacteria. Biosynth’s ProLuciferins are highly effective for the detection of most relevant bacteriastrains, species or groups and can be used in numerous applications, e.g. in foodmonitoring, drinking and recreational water control and microbial diagnostics.

Enzyme activity detection using ProLuciferins:

 


ProLuciferines are easy-to-use. Step 1: the substrates are added to liquid culture media after an enrichment phase. In the presence of target bacteria ProLuciferins are cleaved through bacteria-specific enzyme activity releasing D-luciferin. Step 2: D-Luciferin reacts with the luciferase enzyme contained in the reaction buffer which is added afterwards. The bioluminescent signal is recorded using a luminometer. The intensity of the recorded signal shows a good linear correlation with the bacterial concentration in a given sample. The sensitivity of bioluminescence detections is higher by several orders of magnitude compared to chromogenic bacteria assays. ProLuciferins additionally display a much higher signal-to-noise ratio, especially in comparison with fluorescence.


Workflow of bacteria detection using Biosynth ProLuciferins

Workflow of Bacteria detection using Biosynth ProLuciferins


Products

 

Luc-E.coli K
L-8265

D-Luciferin-6-O-beta-D-glucuronide, dipotassium salt

Luc-E.coli K can be used for the sensitive luminescence detection of E. coli, for sensitive enzyme assays and for GUS reporter gene assays where expression of beta-glucuronidase needs to be monitored. The substrate is typically used at a final concentration of 0.1 mM (20 μL/mL of 5 mM stock solution).



Luc-coliforms
L-8260

Synonyms: D-Luciferin-6-O-beta-D galactopyranoside, Luc-beta-Gal, CAS [131474-38-9]

Luc-coliforms can be used for detection of beta-galactosidase positive microorganisms or for LacZ reporter gene assays. The substrate is used in tests at final concentration 0.11 mM (50 mg/L).



Luc-coliforms Na
L-8263

Synonyms: D-Luciferin-6-O-beta-Dgalacto-pyranoside sodium salt, Luc-beta-Gal, CAS [251903-93-2]

Luc-coliforms Na can be used for detection of beta-galactosidase positive microorganisms and for LacZ reporter gene assays. This sodium form of Luc-coliforms substrates has good solubility in water.



Luc-Salmonella
L-8281

Synonyms: Luc-caprylate

Luc-Salmonella is a synthetic luminogenic substrate for C8-esterase. The enzyme cleaves off caprylate from Luc-Salmonella (L-8281), leading to the release of D-luciferin. Luc-Salmonella can be used for the detection of C8-esterase positive microorganisms such as Salmonella spp. (S.e.:Salmonella enteritidis). Simple one-step C8-esterase assays require only 4 μL/mL of Luc-Salmonella 50X stock solution.



Luc-B.cereus Na
L-8275

Synonyms: D-Luciferin-6-O-choline phosphate, sodium salt

Phosphatidyl-choline specific phospholipase C (PC-PLC) can be found in the Bacillus genus, encoded by the plc, which is regulated by the regulator PlcR. The expression of PlcR is dependent on the growth medium. Luc-B.cereus Na (L-8275) is a synthetic luminogenic substrate for thephosphatidyl-choline specific phospholipase C. This substrate is typically used at 0.1 mM final concentration. Other strains that have PC-PLC activity include Clostridium perfringens, Pseudomonas aeruginosa, Helicobacterpylori, Legionella pneumophila, Bacillus anthracis, Listeria monocytogenes.



Luc-Enterobacteria Na
L-8278

Synonyms: 6-O-(alpha-DGalactopyranosyl) luciferin, sodium salt; Luc-alpha-Gal, sodium salt

Luc-Enterobacteria Na is a synthetic luminogenic substrate that can be used for the detection of Enterobacteria species and other alpha-galactosidase positive microorganisms. Luc-Enterobacteria Na provides high sensitivity and is used at very low final concentration of 0.1 mM.



Luc-phosphate Na
L-8276

Synonyms: D-Luciferin-6-O-phosphat trisodium salt, Luc-P, Luc-Phos, CAS [145613-12-3]

D-Luciferin-6-O-phosphate trisodium salt can be used fordetection of phosphatase positive microorganisms suchas strains of Staphyllococcus or Clostridium in cultivation based assays. The product is typically used at 0.1 mM final concentration.



Luc-Listeria Na
L-8250

Synonyms: D-Luciferin-6-O-myoinositol-1-phosphate, sodium salt

uc-Listeria Na (L-8250) is a synthetic luminogenic substrate for the enzyme Phosphatidylinositol phospholipase C (PI-PLC) that plays an important role insignal transduction processes. Luc-Listeria Na (L-8250) canbe used for the sensitive luminescence detection of PI-PLCpositive strains, for example Listeria monocytogenes. The substrate is typically used at a final concentration of 0.1 mMin bacterial assays.

ProLuciferins for Microbiology

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