Hosta Initial / Callus Medium (HI-1)
    Contains the macro- and micronutrient (Murashige & Skoog) and Vitamins (Linsmaier & Skoog).
    Modified Murashige & Skoog, with (mg/L): 2.0 BAP, 160 Adenine Hemisulfate, 500 Casein and
    0.5 NAA



Component (mg/L)

 Molecular Formula

HI-1

Ammonium Nitrate NH4NO3 1650
Calcium Chloride CaCI2 332.2
Magnesium Sulfate MgSO4 180.7
Potassium Nitrate KNO3 1900
Potassium Phosphate KH2PO4 300
Sodium Phosphate NaH2PO4 170
Boric Acid H3BO3 6.2
Cobalt Chloride CoCI2·6H2O 0.025
Cupric Sulfate CuSO4·5H2O 0.025
Ferric Sodium EDTA C10H12FeN2NaO8·3H2O 42.11
Manganese Sulfate MnSO4·H2O 16.9
Molybdic Acid Na2MoO4·2H2O 0.25
Potassium Iodide KI 0.83
Zinc Sulfate ZnSO4·7H2O 8.6
Glycine C2H5NO2 2
myo-lnositol C6H12O6 100
Thiamine C12H17CIN4OS·HCI 0.4
6-BAP C12H11N5 2
Adenine Hemisulfate (C5H6N5)2SO4·2H2O 160
NAA C12H10O2 0.5
Casein (Enzymatic Hydrolysate)   500
Sucrose C12H22O11 30,000
Agar   8000

Total

 (Grams to make 1L)

43.37

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