Kao & Michayluk Salt Medium (KM-1)
    Contains the macro- and micronutrients (Kao & Michayluk).

Kao & Michayluk Vitamin Solution 100X (KM-2)
    Contains the vitamins (Kao & Michayluk).

Kao & Michayluk Medium (KM-3)
    Contains the macro- and micronutrients and vitamins and organic acids (Kao & Michayluk).
    For Vicia hajastana (similar Pea)
    *Add the following components to KM-3 components (mg/ L) :
    40 Citric Acid(C6H807), 40 Fumarie Acid(C4H404), 40 Maieic Acid(C4H404), 20 Pyruvic
    Acid(C3H3K03)
    0.02 p-Aminobenzoic Acid(C7H7N02), 2 L-Ascorbic Acid(C6H806), 1 Calcium
    Pantothenate(C18H32O10N2Ca)
    1 Choline Chloride(C5H14CINO), 0.4 Folic Aicd(C19H19N706), 0.01 Vitamin D3,
    1 Nicotinamide(C6H6N02)
    0.2 Riboflavin(C17H20N4O6), 0.01 Vitamin A(C20H30O), 0.02 Vitamin B12



Component (mg/L)

 Molecular Formula

KM-1

KM-2

KM-3

Ammonium Nitrate NH4NO3 600   600
Calcium Chloride CaCI2 453   453
Magnesium Sulfate MgSO4 146.55   146.55
Potassium Chloride KCI 300   300
Potassium Nitrate KNO3 1900   1900
Potassium Phosphate KH2PO4 170   170
Boric Acid H3BO3 3   3
Cobalt Chloride CoCI2·6H2O 0.025   0.025
Cupric Sulfate CuSO4·5H2O 0.025   0.025
Ferric Sodium EDTA C10H12FeN2NaO8·3H2O 42.11   42.11
Manganese Sulfate MnSO4·H2O 10   10
Molybdic Acid Na2MoO4·2H2O 0.25   0.25
Potassium Iodide KI 0.75   0.75
Zinc Sulfate ZnSO4·7H2O 2   2
p-Aminbenzoic Acid C7H7NO2   2  
L-Ascorbic Acid C6H8O6   200  
D-Biotin C10H16N2O3S   1 0.01
Calcium Pantothenate C18H32O10N2Ca   100  
Choline Chloride C5H14CINO   100  
Folic Acid C19H19N7O6   40  
myo-lnositol C6H12O6   10,000 100
Nicotinamide C6H6NO2   100  
Pyridoxine C8H11NO3·HCI   100 1
Riboflavin C17H20N4O6   2  
Thiamine C12H17CIN4OS·HCI   100 1
Vitamin A C20H30O   1  
Vitamin B12 C63H88CoN14O14P   2  

Total

 (Grams to make 1L)

3.63

10ml/L

*3.88

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