Linsmaier & Skoog Medium (LS-1)
    Contains the macro- and micronutrients and vitamins (Linsmaier & Skoog).
    For Tobacco & many other species

Linsmaier & Skoog Modified Medium (LS-2)
    Contains the macro- and micronutrients and vitamins (Linsmaier & Skoog).
    With (g/L): 30 Sucrose and 7 Agar.
    For Tobacco & many other species

Linsmaier & Skoog Modified Medium (LS-3)
    Contains the macro- and micronutrients and vitamins (Linsmaier & Skoog).
    With (g/L): 1 MES

Linsmaier & Skoog Modified Medium (LS-4)
    Contains 30 g/L Sucrose, 7 g/L Agar and 1 g/L MES
    Contains the macro- and micronutrients and vitamins (Linsmaier & Skoog).





Component (mg/L)

  Molecular Formula


LS-1


LS-2


LS-3


LS-4


Ammonium Nitrate NH4NO3 1650 1650 1650 1650
Calcium Chloride CaCI2 332.2 332.2 332.2 332.2
Magnesium Sulfate MgSO4 180.7 180.7 180.7 180.7
Potassium Nitrate KNO3 1900 1900 1900 1900
Potassium Phosphate KH2PO4 170 170 170 170
Boric Acid H3BO3 6.2 6.2 6.2 6.2
Cobalt Chloride CoCI2·6H2O 0.025 0.025 0.025 0.025
Cupric Sulfate CuSO4·5H2O 0.025 0.025 0.025 0.025
Ferric Sodium EDTA C10H12FeN2NaO8·3H2O 42.11 42.11 42.11 42.11
Manganese Sulfate MnSO4·H2O 16.9 16.9 16.9 16.9
Molybdic Acid Na2MoO4·2H2O 0.25 0.25 0.25 0.25
Potassium Hydroxide KOH     100 100
Potassium Iodide KI 0.83 0.83 0.83 0.83
Zinc Sulfate ZnSO4·7H2O 8.6 8.6 8.6 8.6
myo-lnositol C6H12O6 100 100 100 100
Thiamine C12H17CIN4OS·HCI 0.4 0.4 0.4 0.4
MES C6H13NO4S     1000 1000
Sucrose C12H22O11   30‘000   30,000
Agar     7000   7000

Total

 (Grams to make 1L)

4.41

41.41

5.51

42.51

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