Tobacco Callus Initial Medium (T-1)
    Contains the macro- and micronutrients and vitamins.
    With (mg/L): 2 IAA and 0.2 Kinetin.

Tobacco Rooting Medium (T-2)
    Contains the macro- and micronutrients and vitamins; without Kinetin.
    With (mg/L): 3 IAA

Tobacco Shooting & Rooting Medium (T-3)
    Contains the macro- and micronutrients and vitamins.
    With (mg/L): 0.03 IAA and 1 Kinetin.

Tobacco Shoot Callus Medium (T-4)
    Contains the macro- and micronutrients and vitamins; without IAA.
    With (mg/L): 1 Kinetin



Component (mg/L)

 Molecular Formula

T-1

T-2

T-3

T-4

Ammonium Nitrate NH4NO3 1650 1650 1650 1650
Calcium Chloride CaCI2 333 333 333 333
Magnesium Sulfate MgSO4 181 181 181 181
Potassium Nitrate KNO3 1900 1900 1900 1900
Potassium Phosphate KH2PO4 170 170 170 170
Boric Acid H3BO3 6.2 6.2 6.2 6.2
Cobalt Chloride CoCI2·6H2O 0.025 0.025 0.025 0.025
Cupric Sulfate CuSO4·5H2O 0.025 0.025 0.025 0.025
Ferric Sodium EDTA C10H12FeN2NaO8·3H2O 42.11 42.11 42.11 42.11
Manganese Sulfate MnSO4·H2O 16.9 16.9 16.9 16.9
Molybdic Acid Na2MoO4·2H2O 0.25 0.25 0.25 0.25
Potassium Iodide KI 0.83 0.83 0.83 0.83
Zinc Sulfate ZnSO4·7H2O 8.6 8.6 8.6 8.6
Glycine C2H5NO2 2 2 2 2
myo-lnositol C6H12O6 100 100 100 100
Nicotinic Acid C6H5NO2 0.5 0.5 0.5 0.5
Pyridoxine C8H11NO3·HCI 0.5 0.5 0.5 0.5
Thiamine C12H17CIN4OS·HCI 0.4 0.4 0.4 0.4
IAA C10H9NO2 2 3 0.03  
Kinetin C10H9N5O 0.2   1 1
Casein   1000 1000 1000 1000

Total

 (Grams to make 1L)

5.41

5.42

5.41

5.41

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