Murashige & Skoog Modified Medium (w/ Vitamins) (MS-4)
    Contains the macro- and micronutrients and vitamins (Murashige & Skoog).
    With (g/L): 30 Sucrose

Murashige & Skoog Modified Medium (w/ Vitamins) (MS-5)
    Contains the macro- and micronutrients and vitamins (Murashige & Skoog).
    With (g/L): 7 Agar and 30 Sucrose

Murashige & Skoog Modified Medium (w/ Vitamins) (MS-10)
    Contains the macro- and micronutrients and vitamins (Murashige & Skoog).
    Modified to contain 500mg/L MES.

Murashige & Skoog Modified Medium (MS-11)
    Contains the macro- and micronutrients (Murashige & Skoog).
    Modified with the addition of (mg/L): 1 BAP and 0.1 NAA.

Murashige & Skoog Modified Medium (MS-12)
    Contains the macro- and micronutrients (Murashige & Skoog).
    Modified with the addition of (mg/L): 30 2iP, 80 Adenine Hemisulfate and 0.3 IAA.

Murashige & Skoog Modified Medium (MS-13)
    Contains the remaining macro- and micronutrients and modified vitamins; without
    Potassium phosphate.
    With (mg/L): 300 Sodium Phosphate Monobasic, 150 Adenine Hemisulfate and 1000 Casein.


Component (mg/L)

Molecular Formula 


MS-4


MS-5


MS-10


MS-11


MS-12


MS-13


Ammonium Nitrate NH4NO3 1650 1650 1650 1650 1650 1650
Calcium Chloride CaCI2 332.2 332.2 332.2 332.2 332.2 332.2
Magnesium Sulfate MgSO4 180.7 180.7 180.7 180.7 180.7 180.7
Potassium Nitrate KNO3 1900 1900 1900 1900 1900 1900
Potassium Phosphate KH2PO4 170 170 170 170 170  
Sodium Phosphate NaH2PO4·H2O         148 300
Boric Acid H3BO3 6.2 6.2 6.2 6.2 6.2 6.2
Cobalt Chloride CoCI2·6H2O 0.025 0.025 0.025 0.025 0.025 0.025
Cupric Sulfate CuSO4·5H2O 0.025 0.025 0.025 0.025 0.025 0.025
Ferric Sodium EDTA C10H12FeN2NaO8·3H2O 42.11 42.11 42.11 42.11 42.11 42.11
Manganese Sulfate MnSO4·H2O 16.9 16.9 16.9 16.9 16.9 16.9
Molybdic Acid Na2MoO4·2H2O 0.25 0.25 0.25 0.25 0.25 0.25
Potassium Iodide KI 0.83 0.83 0.83 0.83 0.83 0.83
Zinc Sulfate ZnSO4·7H2O 8.6 8.6 8.6 8.6 8.6 8.6
Glycine C2H5NO2 2 2 2 2   2
myo-lnositol C6H12O6 100 100 100 100 100 100
Nicotinic Acid C6H5NO2 0.5 0.5 0.5 0.5   5
Pyridoxine C8H11NO3·HCI 0.5 0.5 0.5 0.5   1
Thiamine C12H17CIN4OS·HCI 0.1 0.1 0.1 0.4 0.4 0.5
MES C6H13NO4S     500      
Adenine Hemisulfate (C5H5N5)2SO4·2H2O         80 150
6-BAP C12H11N5       1    
2iP C10H13N5         30  
IAA C10H9NO2         0.3  
NAA C12H10O2       0.1    
Sucrose C12H22O11 30,000 30,000   30,000 30,000  
Casein     7000       1000

Total

 

34.41

41.41

4.91

34.41

34.67

5.7

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