While we keep our proprietary processes as trade secrets, we file patents for our unique application developments. Examples are:
The Aldol® indicator platform is designed to visualize chemical, biochemical or even physical changes in the environment. As such it represents a modern version of traditional indicator systems. However, the Aldol® indicator platform is modular and can be tailored to many applications. For example, it can stain bacteria or change the color of a solution. It can produce fluorescence and a wide range of colors both in solution or as percipitate. Some types of Aldol® dyes readily dissolve in polyethylene or paraffin oil thereby creating bright and permanent fluorescence. Yet another important feature of the Aldol® indicator platform is that the signal (color or fluorescence) is generated without help of oxygen or any other auxiliary agent. It simply responds to a stimulus such as the activation through an enzyme by an intramolecular rearrangement which results in the structure of the dye.
|Modular built||Wide range of applications e.g. chromogenic & fluorogenic indicators|
|No O2 needed for staining||Staining under anaerobic conditions|
|No auxilliary reagents needed||No interference with cell growth|
|Wide color range||complement indigo stains (yellow, organge, red)|
|Stable against unspecific hydrolysis||Aldol ® acetates and other esters can be used in hydrolytic matrices such as ready to use plating media|
|Stain dissolves in hydrophobic phase||Signal can be seperated from sample matrix for instance by staining pipette tip used in assay|
Blood agar plate containing an Aldol® indicator to enhance specificity and sensitivity and allow for fluorescence read-out.
The Aldol® Patent
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Comparison of Resorufin and the common MUG substrate.
From left to right: Resorufin-beta-D-glucuronide, control, Resorufin-beta-D-glucuronide methyl ester, and MUG.
Resorufin-beta-D-glucuronic acid, sodium salt
Resorufin-beta-D-glucuronic acid methyl ester
Biosynth Patent applied:
Many kinds of bacteria produce Phospholipase C (PLC) enzymes. Pathogenic Listeria species for instance secrete inositol specific PLC into the environment. We have developed a whole range of enzyme indicators derived from inositol phosphate including traditional H-MeU, Indolyl as well as advanced Aldol® types.
Choline specific PLC on the other hand is indicative of many Bacillus species. We have developed and patented a number of choline specific PLC substrates using traditional chromophores and fluorogens as well as modern Aldol® versions. These substrates have been used to detect and differentiate Bacillus species as well as to stain B. antracis. Lately, new interest in cholin specific indicators has arisen in the context of Pseudomonas quantification.
Staining of L. monocytogenes (turquois color colony) with X-IP on agar plates.
(Image courtesy of R&F labs)
Aldol® 495 inositol-phosphate, ammonium salt, Biosynth Patent applied: PCT/EP2010/056212
Aldol® 470 choline phosphate, Biosynth Patent applied: PCT/EP2010/056212
Indicators based on the release of indoxyl as a response to an enzymatic stimulus are in wide use. However, current use is limited to a few types of chloro- and bromoindoxyls. Did you know that the fluorinated or iodinated versions of indoxyl indicators offer alternative color schemes (purple) and that you can mix different types of indolyl substrates to produce hetero-indigo dyes yet further extending the color options and intensity?
[2-(2-Thiazolylazo)-4-cresyl]-beta-D-glucuronic acid methyl ester, Biosynth
Patent applied: EP11169147
The presence of bacteria in a sample broth is indicated by turning regular PE beads, added to the sample broth, permanently fluorescent.